The Gunners headed straight to Luton Airport after a training session at London Colney on Wednesday morning.
Unai Emery's men will endure a 1,300 mile trip to Belarus for the first-leg clash at the Borisov Arena.
But the players appeared to be in high spirits as they worked up a sweat at the training ground before take-off.
The Gunners boss watched closely as his side played practice matches and completed a series of drills.
After they all grinned for a cheesy team photograph, the Gunners made their way onto the plane.
Alexandre Lacazette gave fans a glimpse inside the aircraft as he posted a short clip of Alex Iwobi and Ainsley Maitland-Niles to his Instagram page.
Arsenal will be gunning for glory after they were knocked out of last season's competition by Atletico Madrid.
But Emery will fill them with confidence because he has established himself as a Europa League specialist.
The Spaniard led Sevilla to three successive titles before he left to join Paris Saint-Germain in 2016.
Arsenal, who are sixth in the Premier League, enter the clash following this weekend's 2-1 win over Huddersfield.
The Gunners will be reliant on success in the Europa League as their hopes of a top four finish are all but over.
Emery's men were unbeaten in the group stages, with five wins and one draw.
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